anti tlr2  (Proteintech)

Journal: Metabolism Open

Article Title: Deoxycholic Acid and Lipoteichoic Acid cooperatively drive macrophage M2/M1 polarization via TGR5/STAT3 and TLR2/NF-κB to fuel HCC progression in obesity

doi: 10.1016/j.metop.2025.100420

Figure Lengend Snippet: DCA and LTA Treatment Regulate Macrophage Polarization in HFD-fed DEN Mice (A-C)The level of IL-6, IL-1β, and TNF-β in mice liver, n = 3. (D–G) The level of IL-6, IL-10, TGF-β, and TNF-α in liver of mice, n = 3. (H) The gene expression of Arg1, iNOS, CD206, TGR5, TLR2 in liver of mice, n = 3. (I) the protein level of p-NF-κB, p-STAT3, GPC-3 were detected by Western blot, β-actin is an internal parameter. (J) the quantified data of p-NF-κB, p-STAT3, GPC-3 of liver in Mice n = 3. Data are presented as means ± SD. ∗P < 0.05∗∗P < 0.01 ∗∗∗P < 0.001.

Article Snippet: Primary antibody concentrations were: p-NFκB p65 (ab76302, a dilution ratio of 1:1000, Abcam), p-STAT3(bs-1658R, a dilution ratio of 1:1000, Bioss), GPC-3 (30021-1-AP, a dilution ratio of 1:1000, Proteintech), β-actin (66009-1-Ig, a dilution ratio of 1:2000, Proteintech), 53BP1 (ab175933, a dilution ratio of 1:1000, abcam), TLR2 (ab209216, a dilution ratio of 1:1000, Proteintech), TGR5 (ab72608, a dilution ratio of 1:1000, abcam), E-cadherin (ABP0083, a dilution ratio of 1:1000, Abbkine), N-cadherin (22018-1-AP, a dilution ratio of 1:1000, Proteintech), GPC-3 (30021-1-AP, a dilution ratio of 1:1000, Proteintech).

Techniques: Gene Expression, Western Blot

Journal: Metabolism Open

Article Title: Deoxycholic Acid and Lipoteichoic Acid cooperatively drive macrophage M2/M1 polarization via TGR5/STAT3 and TLR2/NF-κB to fuel HCC progression in obesity

doi: 10.1016/j.metop.2025.100420

Figure Lengend Snippet: DCA and LTA treatment promote polarization of Raw264.7 cells towards M2 and M1 types. (A–D) Representative immunofluorescence pictures and quantified data of iNOS and Arg1 in raw264.7 cell, Bar = 50 μm. n = 3. (E–J) The gene expression of TGF-β, IL-10, Arg1, TNF-α, IL-6, and iNOS in raw264.7 cell, n = 3. (K) Representative Western blot pictures and quantified data of TLR2, TGR5, p-NF-Κb, p-STAT3, in raw264.7 cell, β-actin is an internal parameter., n = 3. (P–S) The level of TNF-α, IL-6, IL-10, and TGF-βin supernatant of culture medium of raw264.7 cell, n = 3. Data are presented as means ± SD. ∗P < 0.05∗∗P < 0.01 ∗∗∗P < 0.001.

Article Snippet: Primary antibody concentrations were: p-NFκB p65 (ab76302, a dilution ratio of 1:1000, Abcam), p-STAT3(bs-1658R, a dilution ratio of 1:1000, Bioss), GPC-3 (30021-1-AP, a dilution ratio of 1:1000, Proteintech), β-actin (66009-1-Ig, a dilution ratio of 1:2000, Proteintech), 53BP1 (ab175933, a dilution ratio of 1:1000, abcam), TLR2 (ab209216, a dilution ratio of 1:1000, Proteintech), TGR5 (ab72608, a dilution ratio of 1:1000, abcam), E-cadherin (ABP0083, a dilution ratio of 1:1000, Abbkine), N-cadherin (22018-1-AP, a dilution ratio of 1:1000, Proteintech), GPC-3 (30021-1-AP, a dilution ratio of 1:1000, Proteintech).

Techniques: Immunofluorescence, Gene Expression, Western Blot

Journal: Metabolism Open

Article Title: Deoxycholic Acid and Lipoteichoic Acid cooperatively drive macrophage M2/M1 polarization via TGR5/STAT3 and TLR2/NF-κB to fuel HCC progression in obesity

doi: 10.1016/j.metop.2025.100420

Figure Lengend Snippet: DCA and LTA promote hepatocellular carcinoma cell proliferation and migration by regulating the TGR5 and TLR2 pathways in RAW264.7 cells. (A–G) The gene expression of N-cadherin, Cdkn1a, Cdkn2a, TLR2, TGR5, and E-cadherin in co-cultured hepa1-6 cells. (H–O) Representative Western blot pictures and quantified data of E-cadherin,TLR2, TGR5, p-NF-Κb, GPC3, 53BP1, TLR2, TGR5 in co-cultured hepa1-6 cell, β-actin is an internal parameter., n = 3. (P,Q) Representative pictures and quantified data of migration of co-cultured hepa1-6 cells. (R,S) Representative pictures and quantified data of invasion of co-cultured hepa1-6 cells, Data are presented as means ± SD. ∗P < 0.05∗∗P < 0.01 ∗∗∗P < 0.001.

Article Snippet: Primary antibody concentrations were: p-NFκB p65 (ab76302, a dilution ratio of 1:1000, Abcam), p-STAT3(bs-1658R, a dilution ratio of 1:1000, Bioss), GPC-3 (30021-1-AP, a dilution ratio of 1:1000, Proteintech), β-actin (66009-1-Ig, a dilution ratio of 1:2000, Proteintech), 53BP1 (ab175933, a dilution ratio of 1:1000, abcam), TLR2 (ab209216, a dilution ratio of 1:1000, Proteintech), TGR5 (ab72608, a dilution ratio of 1:1000, abcam), E-cadherin (ABP0083, a dilution ratio of 1:1000, Abbkine), N-cadherin (22018-1-AP, a dilution ratio of 1:1000, Proteintech), GPC-3 (30021-1-AP, a dilution ratio of 1:1000, Proteintech).

Techniques: Migration, Gene Expression, Cell Culture, Western Blot